| 论文题目: | SCO3900, co-transcripted with three downstream genes, is involved in the differentiation of Streptomyces coelicolor. |
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| 作者: | Guohua Zhang, Yuqing Tian, Kun Hu, Chi Feng and Huarong Tan* |
| 联系作者: | 谭华荣 |
| 刊物名称: | Current Microbiology |
| 期: | 4 |
| 卷: | 60 |
| 页: | 268-273 |
| 年份: | 2010 |
| 影响因子: | 1.33 |
| 论文下载: | 下载地址 |
| 摘要: | SCO3900, a putative target gene of the developmental regulator WhiI of Streptomyces coelicolor, encodes a protein similar to PadR from Pediococcus pentosaceus. The SCO3900 disruption mutant exhibited a bald phenotype with sparse aerial hyphae. However, a single copy of SCO3900 under the control of its native promoter could not complement the mutant phenotype. A fragment containing SCO3900 and its downstream three genes completely restored the mutant phenotype to the wild-type. These suggested that the disruption of SCO3900 exerted a polar effect on its downstream genes. The co-transcription of SCO3899, SCO3898, and SCO3897 with SCO3900 was confirmed by the RT–PCR. Moreover, overexpression of SCO3900 in the wild-type strain caused the same phenotype as that of the disruption mutant. The results suggested that SCO3900 encodes a negative regulator participating in morphological differentiation of S. coelicolor by influencing the expression of its downstream genes. |
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