| 论文题目: | Identification of HLA-A24-Binding Peptides of Mycobacterium tuberculosis Derived Proteins with Beta 2m Linked HLA-A24 Single Chain Expressing Cells. |
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| 作者: | Jie Ding Yan Wang,Tingting Cheng, Xiaowei Chen, and Bin Gao* |
| 联系作者: | 高斌 |
| 刊物名称: | Immunological Investigations |
| 期: | 2 |
| 卷: | 39 |
| 页: | 103-113 |
| 年份: | 2010 |
| 影响因子: | 1.754 |
| 论文下载: | 下载地址 |
| 摘要: | Tuberculosis is caused by an intracellular pathogen Mycobacterium tuberculosis (Mtb) and poses a persistent threat to global health. MHC class I-restricted CD8 cytotoxic T lymphocytes (CTL) are essential for protective immunity to Tuberculosis. Information for CTL epitopes derived from Mtb is desirable for vaccine design and assessment of T cell responses. However, the knowledge about CTL epitopes of Mtb, particularly those non-A2 HLA alleles restricted is rare. In this study, beta-2-microglobulin (beta 2m, beta(2)m) linked HLA-A24 single chain was expressed on RMA-S cell line defective in the endogenous antigen processing and applied for screening of peptides which could stabilize the HLA-A24 complex on the cell surface. From a group of peptides predicted as binders by a computer algorithm, five peptides were shown to bind to HLA-A24 protein on the cell surface. As comparison we have also identified a dozen Mtb proteins derived peptides that bind to HLA-A2 specifically. The cell line and HLA binders present here would be useful for further identification of CD8 restricted Mtb epitopes. |
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