| 论文题目: | Proteiniclasticum ruminis gen. nov., sp. nov., a strictly anaerobic proteolytic bacterium isolated from yak rumen |
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| 作者: | Kegui Zhang, Lei Song, and Xiuzhu Dong* |
| 联系作者: | 东秀珠 |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
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| 年份: | 2010 |
| 影响因子: | 2.113 |
| 论文下载: | 下载地址 |
| 摘要: | Two strict anaerobic, proteolytic bacterial strains, designated as strain D3RC-2T and D3RC-3r were isolated from a cellulose-degrading mix-culture enriched from yak rumen content. The strains were Gram stain negative, non-spore-forming with cell size of 0.5-0.8 microm x 0.6-2.0 microm. Growth was observed in the temperature range between 24-46 degrees C (optimum 38-39 degrees C) and pH range between 5.6-8.7 (optimum 7.0-7.3). Two strains used Soja peptone, tryptone, L-phenylalanine, L-leucine, L-methionine, L-serine, L-valine, L-threonine and L-histidine as carbon and nitrogen sources, but did not use any of the tested saccharide. The major fermentation products of PY medium were acetate, propionate and iso-butyrate. The G+C content of strain D3RC-2T and D3RC-3r were 41.0+/-0.1mol% and 41.3+/-0.1mol% (HPLC), respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains represented a new phyletic sub-lineage within the family Clostridiaceae, with <93.8% 16S rRNA gene sequence similarity to the described species. On the basis of phenotypic, genotypic and physiological evidence, strain D3RC-2T and D3RC-3r are proposed as a novel species of a novel genus, for which the name Proteiniclasticum ruminis gen. nov., sp. nov. is proposed. The type strain is D3RC-2T(=AS 1.5057T=JCM 14817T). |
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