| 论文题目: | Protein expression, crystallization and preliminary X-ray crystallographic analysis of chicken interferon-? receptor a chain |
|---|---|
| 作者: | Ping, ZG; Shi, Y; Sun, YL; Ma, LP; Wang, M |
| 联系作者: | Wang, M |
| 刊物名称: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
| 期: | |
| 卷: | 68 |
| 页: | 41-44 |
| 年份: | 2012 |
| 影响因子: | 0.506 |
| 论文下载: | 下载地址 |
| 摘要: | The activity of interferon-gamma (IFN-gamma) relies on signal transduction, which is triggered by combination with the receptors interferon-gamma receptor alpha chain (IFNGR1) and beta chain (IFNGR2). Native recombinant chicken IFNGR1 (chIFNGR1; residues 25237) was overexpressed in Escherichia coli, purified by refolding and crystallized using the vapour-diffusion technique. The crystals belonged to space group P6522, with unit-cell parameters a = b = 64.1, c = 216.3 angstrom, alpha = beta = 90, gamma = 120 degrees. The Matthews coefficient and solvent content were calculated as 2.67 angstrom(3) Da(-1) and 53.97%, respectively. X-ray diffraction data for chIFNGR1 were collected to 2.0 angstrom resolution at a synchrotron source. |
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