| 论文题目: | Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope |
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| 作者: | Yao, SG; Qi, JX; Liu, J; Chen, R; Pan, XC; Li, XY; Gao, F; Xia, C |
| 联系作者: | Xia, C |
| 刊物名称: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
| 期: | |
| 卷: | 68 |
| 页: | 20-23 |
| 年份: | 2012 |
| 影响因子: | 0.506 |
| 论文下载: | 下载地址 |
| 摘要: | In order to clarify the structure and the peptide-presentation characteristics of the equine major histocompatibility complex (MHC) class I molecule, a complex of equine MHC class I molecule (ELA-A1 haplotype, 7-6 allele) with mouse beta 2-microglobulin and the cytotoxic T lymphocyte (CTL) epitope Env-RW12 (RVEDVTNTAEYW) derived from equine infectious anaemia virus (EIAV) envelope protein (residues 195206) was refolded and crystallized. The crystal, which belonged to space group P21, diffracted to 2.3 angstrom resolution and had unit-cell parameters a = 82.5, b = 71.4, c = 99.8 angstrom, beta = 102.9 degrees. The crystal structure contained two molecules in the asymmetric unit. These results should help to determine the first equine MHC class I molecule structure presenting an EIAV CTL epitope. |
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