| 论文题目: | Mc-hES, a novel plasmid carrying human endostatin gene, inhibits nasopharyngeal carcinoma growth |
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| 作者: | Xu, BL; Yuan, L; Wu, JX; Xu, N; Fang, WJ; Zhao, P; Huang, WL |
| 联系作者: | Zhao, P |
| 刊物名称: | CANCER GENE THERAPY |
| 期: | 2 |
| 卷: | 19 |
| 页: | 110-117 |
| 年份: | 2012 |
| 影响因子: | 2.802 |
| 论文下载: | 下载地址 |
| 摘要: | Conventional plasmids for gene therapy produce low-level and short-term gene expression. Here, we first created minicircle carrying endostatin (mc-hES) for measurement of transfection efficiency. Compared with pcDNA-hES, MC-mediated endostatin gene transfer in vitro resulted in seven-fold greater endostatin expression levels in transfected cells and inhibited the growth of Human umbilical vein endothelial cells (HUVEC) more efficiently. HUVEC cell migration and tube-formation assays suggested that MC-mediated endostatin gene has significant anti-migration and anti-tube-formation capacity than that in pcDNA-hES. In vivo experiments showed that after transfection, mc-hES inhibited the growth of nasopharyngeal carcinoma xenografts. The tumor inhibition rates of mc-hES and pcDNA-hES were 60.8% and 26.9%, respectively (P<0.05). MC-mediated intratumoral endostatin expression in vivo was 2.2-17.9 times higher than pcDNA-hES in xenografted mice and lasted for 20 days. Our results suggest that minicircle DNA vectors might be a promising vector for biotherapy and should be further investigated. Cancer Gene Therapy (2012) 19, 110-117; doi: 10.1038/cgt.2011.72; published online 9 December 2011 |
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