| 论文题目: | Bacillus daliensis sp nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake |
|---|---|
| 作者: | Zhai, L; Liao, TT; Xue, YF; Ma, YH |
| 联系作者: | Xue, YF |
| 刊物名称: | INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY |
| 期: | |
| 卷: | 62 |
| 页: | 949-953 |
| 年份: | 2012 |
| 影响因子: | 2.268 |
| 论文下载: | 下载地址 |
| 摘要: | A Gram-positive, alkaliphilic bacterium, designated strain DLS13(T), was isolated from Dali Lake in Inner Mongolia Autonomous Region, China. The isolate was able to grow at pH 7.5-11.0 (optimum at pH 9), in 0-8% (w/v) NaCl (optimum at 2%, w/v) and at 10-45 degrees C (optimum at 30 degrees C). Cells of the isolate were facultatively anaerobic, spore-forming rods with peritrichous flagella. The predominant isoprenoid quinone was MK-7 and its cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acids were anteiso-C-15:0, anteiso-C-17:0 and iso-C-15:0. The genomic DNA G+C content of the isolate was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DLS13(T) was a member of the genus Bacillus and most closely related to Bacillus saliphilus DSM 15402(T) (96.9% similarity). The DNA DNA relatedness value between strain DLS13(T) and B. saliphilus DSM 15402(T) was 38.7 +/- 1.9%. Comparative analysis of genotypic and phenotypic features indicated that strain DLS13(T) represents a novel species of the genus Bacillus, for which the name Bacillus daliensis sp. nov. is proposed; the type strain is DLS13(T) (=CGMCC 1.10369(T)=JCM 17097(T)=NBRC 107572(T)). |
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