| 论文题目: | Ankrd17 positively regulates RIG-I-like receptor (RLR)-mediated immune signaling |
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| 作者: | Wang, YT; Tong, XM; Li, G; Li, JH; Deng, M; Ye, X |
| 联系作者: | Ye, X |
| 刊物名称: | EUROPEAN JOURNAL OF IMMUNOLOGY |
| 期: | 5 |
| 卷: | 42 |
| 页: | 1304-1315 |
| 年份: | 2012 |
| 影响因子: | 5.103 |
| 论文下载: | 下载地址 |
| 摘要: | Retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), such as RIG-I, melanoma differentiation-associated gene 5 (MDA5), and virus-induced signaling adaptor (VISA), are intracellular molecules that sense diverse viral RNAs and trigger immune responses. In this study, we demonstrate that the ankyrin repeat protein ankrd17 interacts with RIG-I, MDA5, and VISA and upregulates RLR-mediated immune signaling. Overexpression of ankrd17 enhances RLR-mediated activation of IRF-3 and NF-?B and upregulates the transcription of IFN-beta. It also promotes RLR signaling in response to poly (I:C), influenza virus RNA, and Sendai virus. Consistently, knockdown of ankrd17 impairs RLR signaling. Furthermore, we demonstrate that ankrd17 enhances the interaction of RIG-I and MDA5 with VISA; the ankyrin repeat domain of ankrd17 is required for its interaction with RIG-I as well as for its function in regulating the RLR pathway. Taken together, our results indicate that ankrd17 is a positive regulator of the RLR signaling pathway. |
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