| 论文题目: | A carnitine-acylcarnitine carrier protein, MoCrc1, is essential for pathogenicity in Magnaporthe oryzae |
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| 作者: | Yang, J; Kong, LG; Chen, XL; Wang, DW; Qi, LL; Zhao, WS; Zhang, Y; Liu, XZ; Peng, YL |
| 联系作者: | Liu, XZ |
| 刊物名称: | CURRENT GENETICS |
| 期: | 3 |
| 卷: | 58 |
| 页: | 139-148 |
| 年份: | 2012 |
| 影响因子: | 2.556 |
| 论文下载: | 下载地址 |
| 摘要: | The rice blast fungus forms a specialized infection structure called an appressorium to breach the host-plant epidermis for successful infection. In this study, a mutant defective in appressorial penetration was isolated by a mutagenesis approach, in which an exogenous DNA fragment was found to be inserted into the first exon of . This gene encodes a putative carnitine-acylcarnitine carrier protein that is widely conserved among eukaryotic organisms. Deletion of severely reduces appressorium turgor generation, appressorial penetration, and development of infection hyphae. The null mutant of lost pathogenicity on intact and abraded host leaves. was also found to be required for growth on minimal medium containing sodium acetate or olive oil. Moreover, the transformed MoCrc1-eGFP fusion protein was expressed throughout the infection process. Our results suggest that the carnitine-acylcarnitine carrier protein plays vital roles in appressorium-mediated infection and is essential for pathogenesis of and perhaps other phytopathogenic fungi. |
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