| 论文题目: | Ectopic Expression of a Bacterium NhaD-type Na+/H+ Antiporter Leads to Increased Tolerance to Combined Salt/Alkali Stresses |
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| 作者: | Zhong, NQ; Han, LB; Wu, XM; Wang, LL; Wang, F; Ma, YH; Xia, GX |
| 联系作者: | Ma, YH |
| 刊物名称: | JOURNAL OF INTEGRATIVE PLANT BIOLOGY |
| 期: | 6 |
| 卷: | 54 |
| 页: | 412-421 |
| 年份: | 2012 |
| 影响因子: | 2.534 |
| 论文下载: | 下载地址 |
| 摘要: | AaNhaD, a gene isolated from the soda lake alkaliphile Alkalimonas amylolytica, encodes a Na+/H+ antiporter crucial for the bacterium's resistance to salt/alkali stresses. However, it remains unknown whether this type of bacterial gene may be able to increase the tolerance of flowering plants to salt/alkali stresses. To investigate the use of extremophile genetic resources in higher plants, transgenic tobacco BY-2 cells and plants harboring AaNhaD were generated and their stress tolerance was evaluated. Ectopic expression of AaNhaD enhanced the salt tolerance of the transgenic BY-2 cells in a pH-dependent manner. Compared to wild-type controls, the transgenic cells exhibited increased Na+ concentrations and pH levels in the vacuoles. Subcellular localization analysis indicated that AaNhaD-GFP fusion proteins were primarily localized in the tonoplasts. Similar to the transgenic BY-2 cells, AaNhaD-overexpressing tobacco plants displayed enhanced stress tolerance when grown in saline-alkali soil. These results indicate that AaNhaD functions as a pH-dependent tonoplast Na+/H+ antiporter in plant cells, thus presenting a new avenue for the genetic improvement of salinity/alkalinity tolerance. |
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