| 论文题目: | The roles of biofilm matrix polysaccharide Psl in mucoid Pseudomonas aeruginosa biofilms |
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| 作者: | Ma, LY; Wang, SW; Wang, D; Parsek, MR; Wozniak, DJ |
| 联系作者: | Ma, LY |
| 刊物名称: | FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY |
| 期: | 2 |
| 卷: | 65 |
| 页: | 377-380 |
| 年份: | 2012 |
| 影响因子: | 2.441 |
| 论文下载: | 下载地址 |
| 摘要: | The opportunistic pathogen Pseudomonas aeruginosa causes life-threatening, persistent infections in patients with cystic fibrosis (CF). Persistence is attributed to the ability of these bacteria to form structured communities (biofilms). Biofilms rely on an extracellular polymeric substances matrix to maintain structure. Psl exopolysaccharide is a key matrix component of nonmucoid biofilms, yet the role of Psl in mucoid biofilms is unknown. In this report, using a variety of mutants in a mucoid P. aeruginosa background, we found that deletion of Psl-encoding genes dramatically decreased their biofilm formation ability, indicating that Psl is also a critical matrix component of mucoid biofilms. Our data also suggest that the overproduction of alginate leads to mucoid biofilms, which occupy more space, whereas Psl-dependent biofilms are densely packed. These data suggest that Psl polysaccharide may have significant contributions in biofilm persistence in patients with CF and may be helpful for designing therapies for P. aeruginosa CF infection. |
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