| 论文题目: | Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4 |
|---|---|
| 作者: | Xu, X; Zhang, XA; Lu, GW; Cai, YP |
| 联系作者: | Cai, YP |
| 刊物名称: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
| 期: | |
| 卷: | 68 |
| 页: | 942-945 |
| 年份: | 2012 |
| 影响因子: | 0.506 |
| 论文下载: | 下载地址 |
| 摘要: | Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.8 angstrom resolution and belonged to space group P21, with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 angstrom, beta = 94.7 degrees. Preliminary analysis of the diffraction data was also performed. |
京公网安备 11010502044263号