| 论文题目: | The function and properties of the transcriptional regulator COS1 in Magnaporthe oryzae |
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| 作者: | Li Xiaoyu, Han Xiuxiu, Liu Zhiqiang, He Chaozu |
| 联系作者: | He Chaozu |
| 刊物名称: | Fungal biology |
| 期: | 4 |
| 卷: | 117 |
| 页: | 239-49 |
| 年份: | 2013 |
| 影响因子: | 1.429 |
| 论文下载: | 下载地址 |
| 摘要: | The conidiophore stalk-less1 (COS1) gene encodes a novel transcription factor in Magnaporthe oryzae, and mutation of COS1 (M2942) resulted in developmental failure of conidiophores. COS1 putatively encodes a 491-amino-acid protein, which contains four multiple adjacent C2H2-type zinc-finger domains. The motifs are homologous to the zinc-finger protein Azf1 of Saccharomyces cerevisiae. Here, we report that the differences of expression profile between M2942 and the wild-type isolate Y34 by RNA-Sequence. DNA sequence analysis of promoter regions of those of COS1-dependent genes showed enrichment in the DNA sequence AAAAGAAA (A4GA3), the putative COS1-binding motif. Gel shift experiments showed that COS1 binds to DNA elements with A4GA3 motif. These suggest that many of the COS1-dependent transcripts may be regulated directly by COS1 binding. |
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