| 论文题目: | Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes |
|---|---|
| 作者: | Zhong Xingyu, Tian Yuqing*, Niu Guoqing, Tan Huarong* |
| 联系作者: | Tian Yuqing*, Tan Huarong* |
| 刊物名称: | Science China. Life sciences |
| 期: | 7 |
| 卷: | 56 |
| 页: | 609-18 |
| 年份: | 2013 |
| 影响因子: | 2.030 |
| 论文下载: | 下载地址 |
| 摘要: | A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters. |
京公网安备 11010502044263号