| 论文题目: | Cloning and characterization of the gene cluster required for beauvericin biosynthesis in Fusarium proliferatum |
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| 作者: | Zhang Tao, Zhuo Ying, Jia Xiaopeng, Liu Jintao, Gao Hong, Song Fuhang, Liu Mei, Zhang Lixin* |
| 联系作者: | Zhang Lixin* |
| 刊物名称: | Science China. Life sciences |
| 期: | 7 |
| 卷: | 56 |
| 页: | 628-37 |
| 年份: | 2013 |
| 影响因子: | 2.030 |
| 论文下载: | 下载地址 |
| 摘要: | Beauvericin, a cyclohexadepsipeptide-possessing natural product with synergistic antifungal, insecticidal, and cytotoxic activities. We isolated and characterized the fpBeas gene cluster, devoted to beauvericin biosynthesis, from the filamentous fungus Fusarium proliferatum LF061. Targeted inactivation of the F. proliferatum genomic copy of fpBeas abolished the production of beauvericin. Comparative sequence analysis of the FpBEAS showed 74% similarity with the BbBEAS that synthesizes the cyclic trimeric ester beauvericin in Beauveria bassiana, which assembles N-methyl-dipeptidol monomer intermediates by the programmed iterative use of the nonribosomal peptide synthetase modules. Differences between the organization of the beauvericin loci in F. proliferaturm and B. bassiana revealed the mechanism for high production of beauvericin in F. proliferatum. Our work provides new insights into beauvericin biosynthesis, and may lead to beauvericin overproduction and creation of new analogs via synthetic biology approaches. |
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