| 论文题目: | Wenyingzhuangia marina gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a recirculating mariculture system |
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| 作者: | Liu Ying, Liu Liang-Zi, Liu Hong-Can, Zhou Yu-Guang, Qi Fang-Jun, Liu Zhi-Pei* |
| 联系作者: | Liu Zhi-Pei* |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
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| 年份: | 2013 |
| 影响因子: | 2.347 |
| 论文下载: | 下载地址 |
| 摘要: | A Gram-staining-negative, strictly aerobic and heterotrophic bacterial strain, designed strain D1T, was isolated from a recirculating mariculture system in Tianjin, China. Its taxonomic position was determined using a polyphasic approach. Cells of strain D1T were non-flagellated short rods with 0.3-0.5 mum wide and 0.5-1.0 mum long. Growth was observed at 15-30 degrees C (optimum, 25 degrees C), at pH 5.5-9.0 (optimum, 6.5-7.0) and in the presence of 1-8 % (w/v) NaCl (optimum, 2-3 %). Cells contained carotenoid pigments but not flexirubin-type pigments. Strain D1T contained MK-6 as the sole menaquinone, and phosphatidylethanolamine (PE) as the sole phospholipid and four unidentified lipids. The major cellular fatty acids (>10 %) were iso-C15:0 (23.2 %), iso-C17:0 3OH (15.2 %), C16:1omega7c/C16:1omega6c (14.3 %), iso-C15:0 3OH (13.5 %) and iso-C15:1 G (10.8 %). 16S rRNA gene sequence analyses indicated that strain D1T belonged to the family Flavobacteriaceae and showed closest phylogenetic relationship to the genus Lutibacter, with highest sequence similarity to Lutibacter aestuarii MA-My1T (92.2 %). The DNA G+C content was 35.9 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain D1T was considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Wenyingzhuangia marina gen. nov., sp. nov. is proposed. The type strain is D1T (=CGMCC 1.12162T =JCM 18494T). |
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