| 论文题目: | Engineering stress tolerance of Escherichia coli by stress-induced-mutagenesis (SIM) based adaptive evolution |
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| 作者: | Zhu Linjiang#, Cai Zhen#, Zhang Yanping, Li Yin* |
| 联系作者: | Li Yin* |
| 刊物名称: | Biotechnol. J. |
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| 年份: | 2013 |
| 影响因子: | 3.446 |
| 论文下载: | 下载地址 |
| 摘要: | Microbial tolerance to toxic products and biomass hydrolysates becomes a challenge for production of fuels and chemicals from renewable resources. To improve cellular tolerance to these environmental stresses, a novel adaptive evolutionary strategy based on stress-induced mutagenesis (SIM) was developed using non-dividing cells. The concept of this method was proved by using Escherichia coli FC40 as a model strain, which was used to quantitatively evaluate the SIM rate. The SIM rate under stressful conditions increased by 92- and 57-fold, respectively, by deleting the mutL or mutS genes to disturb the mismatch repair activity of the host cells. A periodic SIM-based adaptive evolution procedure, which synchronized the mutagenesis and the selection process in a single plate incubation step, was then developed using the mutL-deleting mutant. Using such a method, E. coli mutants tolerant to high concentrations of butanol (13 g/L), NaCl (95 g/L), and high temperature (50 degrees C) were obtained. These results indicate that stress-induced adaptive evolution in non-dividing cells is an effective approach that can improve microbial tolerance against various stresses and generate robust microbial strains suitable for production of fuels and chemicals. |
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