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论文题目:	Structural Diversity of Class 1 Integrons and Their Associated Gene Cassettes in Isolates from a Hospital in China
作者:	Li Bin#, Hu Yongfei#, Wang Qi#, Yi Yong, Woo Patrick, C, Y, Jing Hua, Zhu Baoli, Liu Cuihua*
联系作者:	Liu Cuihua*
刊物名称:	PLoS One
期:	9
卷:	8
页:
年份:	2013
影响因子:	4.244
论文下载:	下载地址
摘要:	BACKGROUND: Klebsiella pneumoniae strains carrying class 1 integrons are becoming more common worldwide, and their role in the dissemination of drug resistance is significant. The aim of this study was to characterize the structural diversity of class 1 integrons and their associated gene cassettes in K. pneumoniae isolates from hospital settings. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed a total of 176 K. pneumoniae isolates in a tertiary-care hospital in Beijing, China for the period of November 1, 2010-October 31, 2011. The presence of class 1 integrons and gene cassettes was analyzed by PCR and sequencing. The prevalence of class 1 integrons was 51.1% (90/176). Fourteen different gene cassettes and 10 different gene cassette arrays were detected. dfrA and aadA cassettes were predominant and cassette combination dfrA1-orfC was most frequently found (13.6%, 24/176). Strong association between resistance to a variety of drugs (both phenotypes and the associated genes) and the presence of class 1 integrons was observed. In addition, we also identified an association between some previously identified prevalent sequence types (such as ST11, ST15, ST147, ST562, and ST716) and the presence of class 1 integrons. CONCLUSIONS/SIGNIFICANCE: Data from this study demonstrated that class 1 integrons are highly diverse and are associated with a variety of drug resistance phenotypes, drug resistance genes, as well as genotypes among K. pneumoniae isolates. Continuous monitoring of gene cassettes in class 1 integrons is warranted to improve the understanding and control of drug resistance among hospital settings.