| 论文题目: | Inhibition effect of enteropeptidase on RANKL-RANK signalling by cleavage of RANK |
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| 作者: | Zhao Yunfeng, Jin Mengmeng, Ma Juan, Zhang Shiqian, Li Wei, Chen Yuan, Zhou Yingsheng, Tao Hong, Liu Yu, Wang Lei, Han Huamin, Niu Ge, Tao Hua, Liu Changzhen*, Gao Bin* |
| 联系作者: | Liu Changzhen*, Gao Bin* |
| 刊物名称: | FEBS Lett |
| 期: | 18 |
| 卷: | 587 |
| 页: | 2958-2964 |
| 年份: | 2013 |
| 影响因子: | 3.478 |
| 论文下载: | 下载地址 |
| 摘要: | Enteropeptidase can cleave trypsinogen on the sequence of Asp-Asp-Asp-Asp-Lys and plays an important role in food digestion. The RANKL-RANK signalling pathway plays a pivotal role in bone remodelling. In this study, we reported that enteropeptidase can inhibit the RANKL-RANK signalling pathway through the cleavage of RANK. A surrogate peptide blocking assay indicated that enteropeptidase could specifically cleave RANK on the sequence NEEDK. Osteoclast differentiation assay and NF-kappa B activity assay confirmed that enteropeptidase could inhibit osteoclastogenesis in vitro through the cleavage of RANK. This is the first study to prove that the RANKL-RANK signalling pathway can be inhibited by cleavage of RANK instead of targeting RANKL. Structured summary of protein interactions: EP cleaves hRANK by cleavage assay (View interaction) EP cleaves mRANK by cleavage assay (View interaction) (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. |
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