| 论文题目: | Methanospirillum psychrodurum sp. nov., isolated from the wetland soil of Qinghai-Tibetan Plateau, China |
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| 作者: | Zhou Liguang, Liu Xiaoli, Dong Xiuzhu* |
| 联系作者: | Dong Xiuzhu* |
| 刊物名称: | International Journal of Systematic and Evolutionary Microbiology |
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| 年份: | 2013 |
| 影响因子: | 2.347 |
| 论文下载: | 下载地址 |
| 摘要: | A psychrotolerant methanogenic strain X-18T, was isolated from the soil of Madoi wetland at Qinghai-Tibetan plateau, China. Cells were wavy rods (11-62 mum long) with blunt tapered ends and Gram-negative stained. Strain X-18T grew strict anaerobically and produced methane exclusively from H2/CO2. Growth occurred in the temperature range of 4-32 degrees C and optimally at 25 degrees C. Growth pH ranged from 6.5-8.0 and optimally at 7.0. The G+C content of the genomic DNA of strain X-18T was 44.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and the alpha subunit of methyl-coenzyme M reductase indicated that strain X-18T affiliated to the genus Methanospirillium, and was most related to Methanospirillum lacunae Ki8-1T at 96.3% 16S rRNA gene sequence similarity. However, strain X-18T was distinguished from the existing Methanospirillium spp. by the lower growth temperature and obligate hydrogenotrophic methanogenesis. On the basis of phenotypic characteristics and phylogenetic analysis, strain X-18T can represent a novel species of the genus Methanospirillum, for which Methanospirillum psychrodurum sp. nov. is proposed and strain X-18T is assigned as the type strain (=CGMCC 1.5186T=JCM 19216T). |
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