| 论文题目: | Coordinated and distinct functions of velvet proteins in Fusarium verticillioides |
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| 作者: | Lan Nan, Zhang Hanxing, Hu Chengcheng, Wang Wenzhao, Calvo Ana, M, Harris Steven, D, Chen She, Li Shaojie* |
| 联系作者: | Li Shaojie* |
| 刊物名称: | Eukaryot Cell |
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| 年份: | 2014 |
| 影响因子: | 3.769 |
| 论文下载: | 下载地址 |
| 摘要: | Velvet-domain-containing proteins are broadly distributed within the fungal kingdom. In the corn pathogen Fusarium verticillioides, previous studies showed that the velvet protein FvVE1 is critical for morphological development, colony hydrophobicity, toxin production and pathogenicity. In this study, tandem affinity purification of FvVE1 revealed that FvVE1 can form a complex with the velvet proteins FvVelB and FvVelC. Phenotypic characterization of gene knockout mutants showed that, as in the case of FvVE1, FvVelB regulated conidial size, hyphal hydrophobicity, fumonisin production and oxidant resistance, while FvVelC was dispensable for these biological processes. Comparative transcriptional analysis of eight genes involved in ROS (reactive oxygen species) removal system revealed that both FvVE1 and FvVelB positively regulated the transcription of a catalase encoding gene FvCAT2. Deletion of FvCAT2 resulted in reduced oxidant resistance, providing further explanation to the regulation of oxidant resistance by velvet proteins in the fungal kingdom. |
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