| 论文题目: | Sulfolobus replication factor C stimulates the activity of DNA polymerase B1 |
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| 作者: | Xing Xuanxuan, Zhang Likui, Guo Li, She Qunxin, Huang Li* |
| 联系作者: | Huang Li* |
| 刊物名称: | J Bacteriol |
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| 年份: | 2014 |
| 影响因子: | 3.586 |
| 论文下载: | 下载地址 |
| 摘要: | Replication factor C (RFC) is known to function in loading proliferating cell nuclear antigen (PCNA) onto primed DNA, allowing PCNA to tether DNA polymerase for highly processive DNA synthesis in eukaryotic and archaeal replication. In this report, we show that RFC complex from the hyperthermophilic archaea of the genus Sulfolobus physically interacts with DNA polymerase B1 (PolB1) and enhances both the polymerase and 3'-5' exonuclease activities of PolB1 in an ATP-independent manner. Stimulation of the PolB1 activity by RFC is independent of the ability of RFC to bind DNA but is consistent with the ability of RFC to facilitate DNA binding by PolB1 through protein-protein interaction. These results suggest that Sulfolobus RFC may play a role in recruiting DNA polymerase for efficient primer extension, in addition to clamp loading, during DNA replication. |
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