| 论文题目: | Photo morphogenesis and Photo response of the blue-light receptor gene Cmwc-1 in different strains of Cordyceps militaris |
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| 作者: | Yang Tao, Dong Caihong* |
| 联系作者: | Dong Caihong* |
| 刊物名称: | FEMS Microbiol Lett |
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| 年份: | 2014 |
| 影响因子: | 2.286 |
| 论文下载: | 下载地址 |
| 摘要: | Light is a necessary environmental factor for stroma formation and development of Cordyceps militaris, a well-known edible and medicinal fungus. In this present study, photo morphogenesis and the blue-light receptor gene were studied using 5 representative strains of C. militaris. The results suggest that light was essential for colony pigmentation and could promote conidia production. Cmwc-1, the homologue of the blue-light photoreceptor of Neurospora crassa, was cloned from the genome of C. militaris by Hi-tail PCR. The protein CmWC-1 was characterized by the presence of the LOV and PAS domains and a GATA-type Znf domain. Genetic variation analysis of Cmwc-1 in different strains showed that 15-bp deletions occurred in 3 strains that resulted in 5-Gln deletions in the transcription activation domain. Phylogenetic analysis based on the Sordariomycetes WC-1-like proteins suggested that the sequence of WC-1 could be used as a candidate marker for phylogenetic analysis in fungi. Cmwc-1 mRNA was light inducible and the expression level increased significantly after irradiation in all tested strains. The sequence of CmWC-1 and the relative expressions responding to irradiation in degenerate and albino strains were similar as the cultivated one. This report will help to open the still-unexplored field of stroma development for this fungus. This article is protected by copyright. All rights reserved. |
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