| 论文题目: | Overexpression of AaPal, a peptidoglycan-associated lipoprotein from Alkalomonas amylolytica, improves salt and alkaline tolerance of Escherichia coli and Arabidopsis thaliana |
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| 作者: | Zhai Lei, Xue Yanfen, Song Yuehao, Xian Mingjie, Yin Liang, Zhong Naiqin, Xia Guixian, Ma Yanhe* |
| 联系作者: | Ma Yanhe* |
| 刊物名称: | Biotechnology Letters |
| 期: | 36 |
| 卷: | 3 |
| 页: | 601-607 |
| 年份: | 2014 |
| 影响因子: | 1.964 |
| 论文下载: | 下载地址 |
| 摘要: | The outer membrane lipoprotein, Pal, plays a major role maintaining the integrity of outer membrane and cell morphology in Gram-negative bacteria. Here, we represent A novel role of AaPal in tolerance to salt and alkaline stresses. The cell density of Escherichia coli expressing AaPal was approx. three times as that of control strain when grown in the presence of 1 M NaCl or at pH 9.0 for 14 h, and transgenic Arabidopsis thaliana grew taller and stronger than wild-type plants when subjected to 200 mM NaCl or pH 9.0 stress. This tolerance was attributed to higher concentrations of K+ and lower concentrations of Na+ in the transgenic organism. Our study provides a potential use of AaPal in the improvement of salt and alkaline tolerance in bacteria and plants. |
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