| 论文题目: | Rheinheimera tuosuensis sp. nov., isolated from a saline lake in Qinghai, China |
|---|---|
| 作者: | Zhong ZhiPing, Liu Ying, Liu LiangZi, Wang Fang, Zhou YuGuang, Liu ZhiPei* |
| 联系作者: | Liu ZhiPei* |
| 刊物名称: | Int J Syst Evol Microbiol |
| 期: | |
| 卷: | |
| 页: | |
| 年份: | 2014 |
| 影响因子: | 2.347 |
| 论文下载: | 下载地址 |
| 摘要: | A Gram-negative bacterium, strain TS-T4T, was isolated from Tuosu Lake, a saline lake (salinity 5.4 %, w/w) in Qaidam basin, Qinghai, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain TS-T4T were non-spore-forming rods, 0.4-0.8 mum wide and 1.7-2.3 mum long, and motile by means of a single polar flagellum. Strain TS-T4T was strictly heterotrophic and aerobic, catalase- and oxidase-positive. Growth was observed in the presence of 0-7.0 % (w/v) NaCl (optimum, 3.0-4.0 %) and at 4-40 degrees C (optimum, 30-35 degrees C) and pH 7.0-10.5 (optimum, pH 8.5-9.0). Strain TS-T4T contained Q-8 as the sole respiratory quinone, and phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as the major polar lipids, just as the other members of the genus Rheinheimera. The predominant fatty acids (>10 %) were summed feature 3 (C16:1 omega7c and/or C16:1 omega6c), C16:0, C17:1 omega8c and C18:1 omega7c. The DNA G+C content was 50.2 mol% (Tm). Phylogenetic trees based on 16S rRNA gene and a conserved portion of gyrase B gene (gyrB) sequences showed that strain TS-T4T was associated with the genus Rheinheimera, and showed the highest 16S rRNA gene sequence similarity to R. longhuensis LH2-2T (97.1 %), then to R. pacifica KMM 1406T (97.0 %). DNA-DNA relatedness of strain TS-T4T to R. longhuensis LH2-2T and R. pacifica KMM 1406T was 53 +/- 2.5 and 48 +/- 2 %, respectively. Based on the data presented above, it is concluded that TS-T4T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera tuosuensis sp. nov. is proposed. The type strain is TS-T4T (=CGMCC 1.12461T= JCM 19264T). |
京公网安备 11010502044263号