| 论文题目: | Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum |
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| 作者: | Zhang Bo, Jiang Cheng-Ying, Liu Yi-Ming, Liu Chang, and Liu Shuang-Jiang*. |
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| 刊物名称: | Biotechnol Lett |
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| 年份: | 2015 |
| 影响因子: | 1.983 |
| 论文下载: | 下载地址 |
| 摘要: | OBJECTIVES: To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum. RESULTS: Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57 %. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l. CONCLUSIONS: A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum. |
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