| 论文题目: | A novel major facilitator transporter TrSTR1 is essential for pentose utilization and involved in xylanase induction in Trichoderma reesei |
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| 作者: | Zhen-Bang* Huang, Xiu-Zhen* Chen, Li-Na* Qin, Hong-Qing* Wu, Xiao-Yun* Su, and Zhi-Yang* Dong. |
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| 刊物名称: | Biochem Biophys Res Commun |
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| 年份: | 2015 |
| 影响因子: | 2.474 |
| 论文下载: | 下载地址 |
| 摘要: | Trichoderma reesei (teleomorph Hypocrea jecorina) is an industrially important filamentous fungus for glycoside hydrolases production, with its xylanolytic enzymes widely applied in many areas. However, the molecular mechanisms underlying xylanase expression are still insufficiently understood. In particular, the effect of sugar transporter on the induction of xylanase expression is unclear. In this work, we identified a novel major facilitator transporter TrSTR1 that is capable of transporting xylose by using a xylose utilization system in Saccharomyces cerevisiae. In T. reesei, TrSTR1 is essential for the utilization of d-xylose, l-arabinose, and even their downstream metabolites D-xylitol and L-arabitol. TrSTR1 is also involved in the induction of xylanase expression since both the xylanase activity and extracellular protein concentration in the Tu6 big up tri, openstr1 strain were decreased, which further confirmed by a qRT-PCR analysis of the transcript levels of the key transcriptional regulators. Our observations provide new insights into connections between pentose utilization and xylanase production in T. reesei. |
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