| 论文题目: | Functional characterization and phylogenetic analysis of acquired and intrinsic macrolide phosphotransferases in the Bacillus cereus group |
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| 作者: | Wang Chao , Sui Zhihai , Leclercq Sebastien Olivier , Zhang Gang , Zhao Meilin , Chen Weiqi , and Feng Jie*. |
| 联系作者: | |
| 刊物名称: | Environmental Microbiology |
| 期: | 17 |
| 卷: | 5 |
| 页: | 1560-1573 |
| 年份: | 2015 |
| 影响因子: | 6.777 |
| 论文下载: | 下载地址 |
| 摘要: | The Bacillus cereus group is composed of Gram-positive spore-forming bacteria of clinical and ecological importance. More than 200 B.cereus group isolates have been sequenced. However, there are few reports of B.cereus group antibiotic resistance genes. This study identified two functional classes of macrolide phosphotransferases (Mphs) in the B.cereus group. Cluster A Mphs inactivate 14- and 15-membered macrolides while Cluster B Mphs inactivate 14-, 15- and 16-membered compounds. The genomic region surrounding the Cluster B Mph gene is related to various plasmid sequences, suggesting that this gene is an acquired resistance gene. In contrast, the Cluster A Mph gene is located in a chromosomal region conserved among all B.cereus group isolates, and data indicated that it was acquired early in the evolution of the group. Therefore, the Cluster A gene can be considered an intrinsic resistance gene. However, the gene itself is not present in all strains and our comparative genomics analyses showed that it is exchanged among strains of the B.cereus group by the mean of homologous recombination. These results provide an alternative mechanism to intrinsic resistance. |
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