| 论文题目: | An Ime2-like mitogen-activated protein kinase is involved in cellulase expression in the filamentous fungus Trichoderma reesei |
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| 作者: | Chen Fei, Chen Xiu-Zhen*, Su Xiao-Yun, Qin Li-Na, Huang Zhen-Bang, Tao Yong, and Dong Zhi-Yang*. |
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| 刊物名称: | Biotechnol Lett |
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| 年份: | 2015 |
| 影响因子: | 1.838 |
| 论文下载: | 下载地址 |
| 摘要: | OBJECTIVES: Eukaryotic mitogen-activated protein kinases (MAPKs) play crucial roles in transducing environmental and developmental signals inside the cell and regulating gene expression, however, the roles of MAPKs remain largely unknown in Trichoderma reesei. RESULTS: T. reesei ime2 (TrIme2) encodes an Ime2-like MAPK in T. reesei. The deletion of the TrIme2 gene led to 90 % increase in cellulase activity against filter paper during earlier period time of cellulase induction as well as the extracellular protein production. Compared to the parent strain, the transcriptional levels of the three major cellulase genes cbh1,cbh2, egl1 were increased by about 9 times, 4 times, 2 times, respectively, at 8 h after cellulase induction in the DeltaTrIme2 mutant. In addition, the disruption of TrIme2 caused over 50 % reduction of the transcript levels of cellulase transcriptional regulators cre1 and xyr1. CONCLUSION: TrIme2 functions in regulation of the expression of cellulase gene in T.reesei, and is a good candidate for genetically engineering of T. reesei for higher cellulase production. |
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