| 论文题目: | A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo |
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| 作者: | Phiwpan Krung, Guo Jie, Zhang Wei, Hu Tanyu, Boruah Bhargavi M, Zhang Jianhua, and Zhou Xuyu*. |
| 联系作者: | |
| 刊物名称: | PLoS One |
| 期: | 10 |
| 卷: | 6 |
| 页: | e0128198 |
| 年份: | 2015 |
| 影响因子: | 3.702 |
| 论文下载: | 下载地址 |
| 摘要: | MicroRNA-155 (miR-155) plays significant role in various physiological processes involving both innate and adaptive immunity. miR-155 expression level changes dynamically during various immune responses. However, current approaches for miR-155 detection at the RNA level do not precisely reflect the real-time activity. Herein, we generated a transgenic mouse line (R26-DTR-155T) for determination of miR-155-5p activity in vivo by inserting miR-155-5p target sequence downstream of a reporter transgene comprising Diphtheria Toxin Receptor and TagBlue fluorescence protein. Using this approach, R26-DTR-155T mice were able to measure variation in levels of miR-155-5p activity in specific cell types of interest. The DTR expression levels were inversely correlated with the endogenous miR-155 expression pattern as detected by quantitative RT-PCR. Our data demonstrate a novel transgenic mouse line which could be useful for tracing miR-155-5p activity in specific cell types through measurement of miR-155-5p activity at single cell level. |
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