| 论文题目: | A primase subunit essential for efficient primer synthesis by an archaeal eukaryotic-type primase |
|---|---|
| 作者: | Liu Bing, Ouyang Songying, Makarova Kira S, Xia Qiu, Zhu Yanping, Li Zhimeng, Guo Li, Koonin Eugene V, Liu Zhi-Jie*, and Huang Li*. |
| 联系作者: | |
| 刊物名称: | Nat Commun |
| 期: | 6 |
| 卷: | |
| 页: | 7300 |
| 年份: | 2015 |
| 影响因子: | 11.904 |
| 论文下载: | 下载地址 |
| 摘要: | Archaea encode a eukaryotic-type primase comprising a catalytic subunit (PriS) and a noncatalytic subunit (PriL). Here we report the identification of a primase noncatalytic subunit, denoted PriX, from the hyperthermophilic archaeon Sulfolobus solfataricus. Like PriL, PriX is essential for the survival of the organism. The crystallographic analysis complemented by sensitive sequence comparisons shows that PriX is a diverged homologue of the C-terminal domain of PriL but lacks the iron-sulfur cluster. Phylogenomic analysis provides clues on the origin and evolution of PriX. PriX, PriL and PriS form a stable heterotrimer (PriSLX). Both PriSX and PriSLX show far greater affinity for nucleotide substrates and are substantially more active in primer synthesis than the PriSL heterodimer. In addition, PriL, but not PriX, facilitates primer extension by PriS. We propose that the catalytic activity of PriS is modulated through concerted interactions with the two noncatalytic subunits in primer synthesis. |
京公网安备 11010502044263号