| 论文题目: | Fermentative production of enantiomerically pure S-1,2-propanediol from glucose by engineered E. coli strain |
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| 作者: | Zhu Lingfeng , Guan Xiangchen , Xie Nengzhong , Wang Limin , Yu Bo* , and Ma Yanhe. |
| 联系作者: | |
| 刊物名称: | Applied Microbiology and Biotechnology |
| 期: | 3 |
| 卷: | 100 |
| 页: | 1241-1251 |
| 年份: | 2016 |
| 影响因子: | 3.848 |
| 论文下载: | 下载地址 |
| 摘要: | The pure stereoisomers of 1,2-propanediol (1,2-PDO) could be used as starting materials to synthesize high value-added specialty chemicals and chiral pharmaceutical products. As the stereoisomers of 1,2-PDO cannot be obtained by traditional chemical synthesis processes, biotechnological processes have gained increasing attention. However, to our knowledge, the production of S-1,2-PDO directly from glucose has not been previously reported. In this study, we demonstrate a novel artificial pathway to convert L-lactic acid to S1,2- PDO and its integration into the genome of Escherichia coli strain BW25113 Delta poxB with synchronous deletion of genes responsible for branch metabolic pathways from glucose. L-lactate production was increased by replacing the native D-lactate dehydrogenase with the L-lactate dehydrogenase from Bacillus coagulans. The methylglyoxal bypass pathway was blocked to avoid synthesis of a racemic mixture of D-and L-lactate and prevent the accumulation of methylglyoxal, a toxic intermediate. To further improve the yield of S-1,2PDO, a novel cofactor regeneration system was introduced by combining pyruvate decarboxylase and acetaldehyde-CoA dehydrogenase II to simultaneously regenerate NADH and the CoA donor of acetyl-CoA for the lactate conversion pathway. Finally, 13.7 mM S-1,2-PDO with >99 % enantiomeric purity was directly produced from glucose by disrupting the major carbon-competing pathways and strengthening the lactate transformation pathway. This study demonstrates the first attempt to synthesize S-1,2-PDO by direct fermentation of glucose. |
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