| 论文题目: | Ligand determinants of nisin for its induction activity |
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| 作者: | Ge Xiaoxuan, Teng Kunling*, Wang Jian, Zhao Fangyuan, Wang Fangfang, Zhang Jie, and Zhong Jin*. |
| 联系作者: | |
| 刊物名称: | Journal of dairy science |
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| 年份: | 2016 |
| 影响因子: | 3.071 |
| 论文下载: | 下载地址 |
| 摘要: | Nisin has been widely used in the food industry as a safe and natural preservative and has the potential to be used as a biomedicine. Improving nisin production is important for its enormous applications. Nisin A is produced in Lactococcus lactis and its biosynthesis is induced through the regulation of the 2-component system NisKR. In this study, alanine-scanning mutagenesis was applied to study the key structure or AA in nisin for inducing the 2-component system NisKR to regulate downstream gene expression. Assay of beta-galactosidase activity revealed that either ring A or ring B was necessary for nisin to induce lacZ reporter gene expression. A substituted first ring formed by Thr2 and Cys7 in S3A instead of ring A (formed by Ser3 and Cys7) fully retained nisin induction activity. Mutation of cationic AA and addition of cationic ions hardly affected nisin induction activity. These results demonstrated that the N-terminal ring structures in nisin were involved in activating NisKR to act as an inducing molecule, whereas the electrostatic force might not contribute to this process. In addition, 2 specific residues were revealed to have potential for improving both nisin induction and antimicrobial activity, which might be used for increasing nisin production. |
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