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科研成果

论文题目:	Aquisalinus flavus gen. nov., sp nov., a member of the family Parvularculaceae isolated from a saline lake
作者:	Zhong Zhi-Ping , Liu Ying , Wang Fang , Zhou Yu-Guang , Liu Hong-Can , and Liu Zhi-Pei*
联系作者:
刊物名称:	International Journal of Systematic and Evolutionary Microbiology
期:
卷:	66
页:	1813-1817
年份:	2016
影响因子:	2.403
论文下载:	下载地址
摘要:	A Gram-stain-negative bacterium, strain D11M-2(T), was isolated from a saline lake (Lake Dasugan) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells were non-spore-forming rods, 0.5-0.7 mu m wide and 1.2-1.6 mu m long, and motile by means of a single subpolar or lateral flagellum. Strain D11M-2(T) was strictly heterotrophic and aerobic, and catalase-and oxidase-positive. Growth was observed in the presence of 0-14.0 % (w/v) NaCl (optimum, 2.0 %), and at 10-35 degrees C (optimum, 30 degrees C) and pH 6.0-10.5 (optimum, pH 8.0). Strain D11M-2(T) contained Q-10 and Q-11 as the respiratory quinones and three unknown glycolipids as the major polar lipids. The major cellular fatty acids (>10.0 %) were summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c) and C-16 : 0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D11M-2(T) belonged to the family Parvularculaceae and formed a separate lineage that was independent of the two genera within the family Parvularculaceae. Strain D11M-2(T) exhibited 92.8-93.4 % 16S rRNA gene sequence similarity to members of the genus Parvularcula (highest to Parvularcula bermudensis HTCC 2503(T)), and 90.2 % to a member of the genus Amphiplicatus. The DNA G+C content was 59 mol% (T-m). Based on the phenotypic, chemotaxonomic and phylogenetic data, strain D11M-2(T) is considered to represent a novel species of a new genus in the family Parvularculaceae, for which the name Aquisalinus flavus gen. nov., sp. nov. is proposed. The type strain of Aquisalinus flavus is D11M-2(T) (=CGMCC 1.12921(T) =KCTC 42673(T)).