| 论文题目: | Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteria |
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| 作者: | Zhou, Jie Zhang, Fuliang Meng, Hengkai Zhang, Yanping Li, Yin |
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| 刊物名称: | Metab Eng |
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| 年份: | 2016 |
| 影响因子: | 8.322 |
| 论文下载: | 下载地址 |
| 摘要: | Increasing photosynthetic efficiency is crucial to increasing biomass production to meet the growing demands for food and energy. Previous theoretical arithmetic analysis suggests that the light reactions and dark reactions are imperfectly coupled due to shortage of ATP supply, or accumulation of NADPH. Here we hypothesized that solely increasing NADPH consumption might improve the coupling of light reactions and dark reactions, thereby increasing the photosynthetic efficiency and biomass production. To test this hypothesis, an NADPH consumption pathway was constructed in cyanobacterium Synechocystis sp. PCC 6803. The resulting extra NADPH-consuming mutant grew much faster and achieved a higher biomass concentration. Analyses of photosynthesis characteristics showed the activities of photosystem II and photosystem I and the light saturation point of the NADPH-consuming mutant all significantly increased. Thus, we demonstrated that introducing extra NADPH consumption ability is a promising strategy to increase photosynthetic efficiency and to enable utilization of high-intensity lights. |
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