| 论文题目: | Activation and Identification of NC-1: A Cryptic Cyclodepsipeptide from Red Soil-Derived Streptomyces sp FXJ1.172 |
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| 作者: | Liu, Minghao Liu, Ning Shang, Fei Huang, Ying |
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| 刊物名称: | European Journal of Organic Chemistry |
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| 年份: | 2016 |
| 影响因子: | 2.971 |
| 论文下载: | 下载地址 |
| 摘要: | Genome sequence analysis of a lasalocid-producing strain, Streptomyces sp. FXJ1.172, which was isolated from red soil, indicated its great untapped biosynthetic potential for novel secondary metabolites. A lasalocid-null mutant Streptomyces sp. FXJ1.172lodS, was then constructed to make way for the cryptic biosynthetic pathways. Consequently, a novel cyclodepsipeptide, named NC-1, was activated and isolated from the lasalocid abolished mutant strain cultivated in glucose-yeast extract-malt extract (GYM) medium supplemented with ferric ion. The planar- and stereo-structures of NC-1 were elucidated based on detailed analyses of MS, NMR, and single-crystal X-ray diffraction data, revealing it as the first reported cyclodepsipeptide that is epoxidized at adjacent carbon atoms of the cinnamoyl fatty acid side chain. In assays of antimicrobial activity, NC-1 exhibited moderate inhibition effects against Mycobacterium bovis bacille Calmette-Guerin (BCG). |
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