| 论文题目: | Engineering deacetoxycephalosporin C synthase as a catalyst for the bioconversion of penicillins |
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| 作者: | Fan, Keqiang Lin, Baixue Tao, Yong Yang, Keqian |
| 联系作者: | Yang, Keqian* |
| 刊物名称: | J Ind Microbiol Biotechnol |
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| 年份: | 2016 |
| 影响因子: | 2.732 |
| 论文下载: | 下载地址 |
| 摘要: | 7-aminodeacetoxycephalosporanic acid (7-ADCA) is a key intermediate of many clinically useful semisynthetic cephalosporins that were traditionally prepared by processes involving chemical ring expansion of penicillin G. Bioconversion of penicillins to cephalosporins using deacetoxycephalosporin C synthase (DAOCS) is an alternative and environmentally friendly process for 7-ADCA production. Arnold Demain and co-workers pioneered such a process. Later, protein engineering efforts to improve the substrate specificity and catalytic efficiency of DAOCS for penicillins have been made by many groups, and a whole cell process using Escherichia coli for bioconversion of penicillins has been developed. |
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