| 论文题目: | Microbacterium sorbitolivorans sp. nov., a novel member of Microbacteriaceae isolated from fermentingbed in pigpen |
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| 作者: | Cai Man, Meng Yu-Chen, Liu Hong-Can, Yang Lei-Lei, Kang Ying-Qian, and Zhou Yu-Guang*. |
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| 刊物名称: | Int J Syst Evol Microbiol |
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| 年份: | 2016 |
| 影响因子: | 2.782 |
| 论文下载: | 下载地址 |
| 摘要: | Two bacterial strains SZDIS-1-1T and GZDIS-1-1 were isolated from fermentingbed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short-rods without flagellum. Their nearest phylogenetic neighbors were Microbacterium amylolyticum N5T (16S rRNA gene sequences similarity 98.2 %), Microbacterium indicum BBH6T (97.9 %) and Microbacterium gubbeenense DPC 5286T (97.8 %) with DNA-DNA hybridization values to strain SZDIS-1-1T as 20.0+/-1.2 %, 14.3+/-5.8 % and 19.1+/-1.6 %, respectively. The DNA G+C content of the new isolates were 67.6-71.1 mol %, and C15 : 0 anteiso, C17 : 0 anteiso, C16 : 0 iso were their predominant cellular fatty acids, which were consistent with classification in genus Microbacterium. However, cell-wall sugars and characteristic amino acid were rhamnose, glucose, galactose and ornithine. Major menaquinones were MK-11 and MK-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipids, unknown phospholipid, and unknown lipids. These characteristics constructed distinct profile of the two isolates. Therefore, based on polyphasic taxonomic characteristics, strains SZDIS-1-1T and GZDIS-1-1 represented a novel species of genus Microbacterium, for which the name Microbacterium sorbitolivorans sp. nov. is proposed, with strain SZDIS-1-1T (= CGMCC 1.15228T= DSM 103422T) as the type strain. |
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