| 论文题目: | Direct and tunable modulation of protein levels in rice and wheat with a synthetic small molecule |
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| 作者: | Zhang Jingbo, Yin Kangquan, Sun Juan, Gao Jinlan, Du Qiuli, Li Huali, and Qiu Jin-Long*. |
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| 刊物名称: | Plant Biotechnol J |
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| 年份: | 2017 |
| 影响因子: | 6.657 |
| 论文下载: | 下载地址 |
| 摘要: | Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system is applicable to economically important crops. In this study, we show that the RDDK-Shld1 system enables rapid and tunable control of protein levels in rice and wheat. Accumulation of RDDK fusion proteins can be reversibly and spatio-temporally controlled by the synthetic small-molecule Shld1. Moreover, RDDK-Bar and RDDK-Pid3 fusions confer herbicide and rice blast resistance, respectively, in a Shld1-dependent manner. Therefore, the RDDK-Shld1 system provides a reversible and tunable technique for controlling protein functions and conditional expression of transgenes in crops. |
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